1. Your Application
  2. Cell Disruption
  3. Protein & DNA Extraction
  4. DNA Manipulation
  5. Enzyme Release
  6. Selective Breakage
  7. Cell Isolation
  8. Tissue Disruption

Your Application

Our range of high pressure cell disrupters offer a highly reliable and flexible method of cell disruption for your specialist requirements. We are happy to advise you on how our products may fit into your production needs or research agenda. Just a few examples of cells that have been successfully processed by Constant Systems Limited may be viewed on the Cell Success Page. New applications are continually being discovered.

Constant Systems Cell Disrupters experience with E.coli and Yeast is vast and breakage depends on the cell culture conditions, length of growth cycle and pressure utilised. However Constant Systems Ltd recommend the following as a guide to users for pressure compared to breakage.

 

E.coli Disruption & Guidance List

Below 15,000 psi
(1000 Bar)

-Release of DNA

15,000 psi - 20,000 psi
(1350 Bar)

-Cytoplasmic Protein

20,000 psi - 27,000 psi
(1900 Bar)

-Inclusion Bodies

27,000 psi - 35,000 psi
(2400 Bar)

-Membrane Protein
E-Coli

Yeast Disruption & Guidance List

Yeast

Species

-Optimal Pressure

Saccharomyces Cerevisiae

-27 - 30Kpsi

Candida Albicans

-25 - 40Kpsi

Candida Cloacae

-40Kpsi

Candida Utilis

-5 - 40Kpsi

Hansenula Polymorpha

-40Kpsi

Kluyveromyces Fragilis

-5 - 40Kpsi

Pichia Augusta

-40Kpsi

Pichia Picorna

-30Kpsi

Pichia Pastoris

-40Kpsi

Pichia Capsulatus

-30Kpsi

Rhodotorula Glutinis

-40Kpsi

Schizosaccharomyces Pombe

-35 - 40Kpsi

The above information is provided by Constant Systems Limited as a guide based on previous experimental work carried out with Constant Systems Cell Disrupters. Constant Systems considers this information as confidential and accepts no liability for its use.

Your Application

Cell disruption is no longer the hit and miss field that it used to be, and Constant Systems Ltd have developed a process that is reliable and repeatable.

Cell Disruption

General multi-purpose cell disruption can be carried out with Constant Cell Disruption Systems on a small research scale or in pilot and production scale facilities. Disruption of sub cellular and intracellular particles from one pass is possible over a wide range of organisms which include algae, animal cells, bacteria, environmental samples, fungi (inc yeasts), mammalian cells and tissue, parasites, plant cells and tissues, viruses.

 Sele

Free Flowing Sample Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the Continuous Flow Models.

Viscous Samples Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the HAIVA Models.

Solid Sample Types (Plant, Tissue, Frozen samples)
For processing Solid Sample Types then please view the One Shot model or the One Shot Head Adaptor *

NOTE * One Shot Head Adaptor can only be used with a Constant Systems Cell Disrupter and can be fitted to the TS, Z, HAIVA, B or the C series range

Your Application

Protein and DNA extraction is a common method of obtaining important cell components at both research and production levels. Constant Cell Disruption Systems are used widely for a number of organisms.

Protein & DNA Extraction

Results from one pass include:

  • '100% protein release from saccharomyces cerevisiae'

  • 'High protein recovery from mycobacterium smegmatis and e.coli'

  • 'DNA extraction from strawberry leaves and soil bacteria'

 Protein

Free Flowing Sample Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the Continuous Flow Models.

Viscous Samples Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the HAIVA Models.

Solid Sample Types (Plant, Tissue, Frozen samples)
For processing Solid Sample Types then please view the One Shot model or the One Shot Head Adaptor *

NOTE * One Shot Head Adaptor can only be used with a Constant Systems Cell Disrupter and can be fitted to the TS, Z, HAIVA, B or the C series range

Your Application

DNA manipulation is a growing area of cell research and Constant Cell Disruption Systems are able to break DNA randomly and to particular lengths in order for cloning to be carried out.

DNA Manipulation

Results from one pass include:

  • 'Manipulation of yeast chromosomal DNA'

  • 'DNA from a strain of e.coli'

 DNA

Free Flowing Sample Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the Continuous Flow Models.

Viscous Samples Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the HAIVA Models.

Solid Sample Types (Plant, Tissue, Frozen samples)
For processing Solid Sample Types then please view the One Shot model or the One Shot Head Adaptor *

NOTE * One Shot Head Adaptor can only be used with a Constant Systems Cell Disrupter and can be fitted to the TS, Z, HAIVA, B or the C series range

Your Application

Enzyme release is often carried out at research and production levels. Constant Cell Disruption Systems are used for efficient release from a number of organisms.

Enzyme Release

Results from one pass include:

  • 'Excellent enzyme activity from recombinant e.coli'

  • 'High specific activity of enzyme from aspergillus nidulans'

 Enzyme

Free Flowing Sample Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the Continuous Flow Models.

Viscous Samples Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the HAIVA Models.

Solid Sample Types (Plant, Tissue, Frozen samples)
For processing Solid Sample Types then please view the One Shot model or the One Shot Head Adaptor *

NOTE * One Shot Head Adaptor can only be used with a Constant Systems Cell Disrupter and can be fitted to the TS, Z, HAIVA, B or the C series range

Your Application

The flexibility of Constant Cell Disruption Systems allows a wide range of cell components to be disrupted and reproducible results to be obtained. Selective disruption lies in the release of intact organelles from disrupted cells.

Selective Breakage

of selective breakage from one pass include:

  • 'Separation of nucleus and membrane in amniotic cells'

  • 'Selective breakage of chicken sperm'

  • 'Chloroplasts from euglena gracillis'

 Cell

Free Flowing Sample Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the Continuous Flow Models.

Viscous Samples Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the HAIVA Models.

Solid Sample Types (Plant, Tissue, Frozen samples)
For processing Solid Sample Types then please view the One Shot model or the One Shot Head Adaptor *

NOTE * One Shot Head Adaptor can only be used with a Constant Systems Cell Disrupter and can be fitted to the TS, Z, HAIVA, B or the C series range

Your Application

Single cell isolation is most common with tissue work and the flexibility of Constant Cell Disruption System means that viable single cells can be separated with little damage.

Single Cell Isolation

Results from one pass include:

  • 'Isolation of viable single cells from calf spleen and lymph nodes'

 Single Cell

Free Flowing Sample Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the Continuous Flow Models.

Viscous Samples Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the HAIVA Models.

Solid Sample Types (Plant, Tissue, Frozen samples)
For processing Solid Sample Types then please view the One Shot model or the One Shot Head Adaptor *

NOTE * One Shot Head Adaptor can only be used with a Constant Systems Cell Disrupter and can be fitted to the TS, Z, HAIVA, B or the C series range

Your Application

Mammalian and plant tissue is often awkward to handle but Constant Cell Disruption Systems have been successful in disrupting a variety of tissues.

Tissue Disruption

Results from one pass include:

  • 'Disruption of calf, ox, pig and horse liver'

  • 'Extraction of pectin from banana fruit'

  • 'Disruption of spinach, arabidoposis and strawberry leaves'

 Tissue

Free Flowing Sample Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the Continuous Flow Models.

Viscous Samples Types
For processing volumes of 20mls or less then please view the One Shot model or the One Shot Head Adaptor *
For processing volumes greater than 20mls then please view the HAIVA Models.

Solid Sample Types (Plant, Tissue, Frozen samples)
For processing Solid Sample Types then please view the One Shot model or the One Shot Head Adaptor *

NOTE * One Shot Head Adaptor can only be used with a Constant Systems Cell Disrupter and can be fitted to the TS, Z, HAIVA, B or the C series range