Constant Systems is
registered and approved by British Standards Institute
ISO 9001:2000
FS67550
Your Application
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Our range of high pressure cell disrupters offer a highly reliable and flexible method of cell disruption for your specialist requirements. We are happy to advise you on how our products may fit into your production needs or research agenda. Just a few examples of cells that have been successfully processed by Constant Systems Limited may be viewed on the Cell Success Page. New applications are continually being discovered.
Constant Systems Cell Disrupters experience with E.coli and Yeast is vast and breakage depends on the cell culture conditions, length of growth cycle and pressure utilised. However Constant Systems Ltd recommend the following as a guide to users for pressure compared to breakage.
E.coli Disruption & Guidance List
Below 15,000 psi (1000 Bar)
-Release of DNA
15,000 psi - 20,000 psi (1350 Bar)
-Cytoplasmic Protein
20,000 psi - 27,000 psi (1900 Bar)
-Inclusion Bodies
27,000 psi - 35,000 psi (2400 Bar)
-Membrane Protein
Yeast Disruption & Guidance List
Species
-Optimal Pressure
Saccharomyces Cerevisiae
-27 - 30Kpsi
Candida Albicans
-25 - 40Kpsi
Candida Cloacae
-40Kpsi
Candida Utilis
-5 - 40Kpsi
Hansenula Polymorpha
-40Kpsi
Kluyveromyces Fragilis
-5 - 40Kpsi
Pichia Augusta
-40Kpsi
Pichia Picorna
-30Kpsi
Pichia Pastoris
-40Kpsi
Pichia Capsulatus
-30Kpsi
Rhodotorula Glutinis
-40Kpsi
Schizosaccharomyces Pombe
-35 - 40Kpsi
The above information is provided by Constant Systems Limited as a guide based on previous experimental work carried out with Constant Systems Cell Disrupters. Constant Systems considers this information as confidential and accepts no liability for its use.
